Beszerzési és Szolgáltatási Iroda (anyagok, eszközök)
Call for Proposal No. SZTE/2014/PB04022|
OxiSelectTM HNE Adduct Competitive ELISA Kit Manufacturer: Cell Biolabs, Inc. STA-838 96 assays Quantity: 1 |
Detailed specification
Assay Principle
First, an HNE conjugate is coated on an ELISA plate. The unknown HNE protein samples or HNE-BSA standards are then added to the HNE conjugate preabsorbed ELISA plate. After a brief incubation, an anti-HNE polyclonal antibody is added, followed by an HRP conjugated secondary antibody. The content of HNE protein adducts in unknown samples is determined by comparison with a predetermined HNE-BSA standard curve.
Kit Components
Box 1 (shipped at room temperature)
1. 96-well Protein Binding Plate (Part No. 231001): One strip well 96-well plate (8 x 12).
2. Anti-HNE Antibody (1000X) (Part No. 283801): One 10 μL vial of anti-HNE antibody.
3. Secondary Antibody, HRP Conjugate (1000X) (Part No. 231704): One 20 μL vial.
4. Assay Diluent (Part No. 310804): One 50 mL bottle.
5. 10X Wash Buffer (Part No. 310806): One 100 mL bottle.
6. Substrate Solution (Part No. 310807): One 12 mL amber bottle.
7. Stop Solution (Part. No. 310808): One 12 mL bottle.
Box 2 (shipped on blue ice packs)
1. HNE-BSA Standard (Part No. 283803): One 250 μL vial of 1 mg/mL HNE-BSA in PBS.
2. HNE Conjugate (Part No. 283802): One 50 μL vial of HNE conjugate at 1.0 mg/mL in PBS.
3. 100X Conjugate Diluent (Part No. 281603): One 300 μL vial.
Storage
Upon receipt, aliquot and store the Anti-HNE Antibody, HNE-BSA Standard, HNE Conjugate and 100X Conjugate Diluent at -20ºC to avoid multiple freeze/thaw cycles. Store all other kit components at 4ºC.
Winner supplier: THP Medical Products Vertriebs GmbH
Winner price: 210 000 HUF + VAT
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