"Discovery of cancer-relevant genes and pathways using transposon-based forward genetic screens and targeted nucleases" címmel, az American Cancer Society kutató professzora - Prof. David A. Largaespada - tartja előadását
"Understanding the complexity of genomic and epigenetic alterations in human cancer remains a daunting challenge.
We have developed an approach to define strong candidate cancer genes in mouse models using unbiased, forward genetic screens based on transposon insertional mutagenesis using the Sleeping Beauty (SB) transposon system.
We recently reported the results of a screen for novel drivers of neurofibroma and malignant peripheral nerve sheath tumor (MPNST) development (Rahrmann et al., Nature Genet., 2013). We discovered novel MPNST genes and genetic pathways and validated some of them using TAL endonuclease-mediated genetic manipulation of human MPNST or Schwann cell lines.
We have completed a similar screen for drivers of osteosarcoma (OS) development. Mutagenesis induced OS in wild type mice and accelerated it on a Trp53 deficient background. Over 200 candidate genes were identified, many of which are altered in human cancers including OS.
Signaling pathways enriched for candidate genes were also identified and a subset of these pathways and genes were functionally validated, using targeted nuclease modification of human immortalized osteoblasts, and represent new targets for OS treatment."
Szeretettel várnak minden érdeklődőt: 2015. április 15.-én, szerdán, 10:00 órakor.
